FIGURE
Fig. 5
- ID
- ZDB-FIG-100107-43
- Publication
- Christiansen et al., 2009 - Critical early roles for col27a1a and col27a1b in zebrafish notochord morphogenesis, vertebral mineralization and post-embryonic axial growth
- Other Figures
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Fig. 5
col27a1a is required for notochord development. Brightfield analysis of morphant (B) and mismatch control (A) notochords at ∼4.2 SSL (8 dpf) showed buckling of the notochord sheath. Dorsal (D) and lateral (F) views of col27a1a morphants at ∼5.6 SSL (14 dpf) show formation of notochord curves at the distal end of the tail. (C, E, G) Mismatch control siblings. (H) Calcein staining to visualize mineral showed abnormal vertebrae (white arrow) and decreased mineralization (white bar indicates unmineralized region). Scale bar in A represents 20 μm and applies to B. Scale bar in C represents 200 μm and applies to D–H. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Knockdown Reagents: | |
| Observed In: | |
| Stage Range: | Day 6 to Days 7-13 |
Phenotype Detail
Acknowledgments
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