FIGURE

Fig. 6

ID

ZDB-FIG-100107-44

Publication

Christiansen et al., 2009 - Critical early roles for col27a1a and col27a1b in zebrafish notochord morphogenesis, vertebral mineralization and post-embryonic axial growth

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Fig. 6

Protein and cellular accumulation in the col27a1a morphant notochord.

Semithin sections (300 nm) of mismatch control (A) and col27a1a morphant (B) notochord were stained with Richardson′s stain for proteins at ∼4.5 SSL (10 dpf). The morphant notochord showed abnormal cellular clumping along the notochord sheath (white box and inset) and increased staining along membranes between notochord cells. (C–F) TEM analysis indicated that this staining was due to increased protein accumulation along membranes separating notochord cells. Scale bar in A represents 200 μm and applies to B. Scale bar in A inset represents 20 μm and applies to B inset. Scale bars in C and E represent 2 μm and apply to D and F, respectively. s, notochord sheath; sc, sheath cell; m, membrane.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-col27a1a + MO2-col27a1a
Knockdown Reagents:	MO1-col27a1a MO2-col27a1a
Observed In:	notochord notochord development
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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