Fig. 5 col27a1a is required for notochord development.
Brightfield analysis of morphant (B) and mismatch control (A) notochords at ∼4.2 SSL (8 dpf) showed buckling of the notochord sheath. Dorsal (D) and lateral (F) views of col27a1a morphants at ∼5.6 SSL (14 dpf) show formation of notochord curves at the distal end of the tail. (C, E, G) Mismatch control siblings. (H) Calcein staining to visualize mineral showed abnormal vertebrae (white arrow) and decreased mineralization (white bar indicates unmineralized region). Scale bar in A represents 20 μm and applies to B. Scale bar in C represents 200 μm and applies to D–H.