FIGURE

Fig. 7

ID

ZDB-FIG-090923-15

Publication

Zhao et al., 2009 - Labelling and targeted ablation of specific bipolar cell types in the zebrafish retina

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Fig. 7

Prodrug dependent ablation of specific bipolar cell types in larvae from enhancer trap lines. (A-H) Confocal images of retinal cross-sections from larvae (5 dpf) produced by mating of UAS:nfsB-mCherry transgenic fish to Tg(Gal4-VP16;UAS:eGFP)^xfz3. (A, B) Retina from a larva without the UAS:nfsB-mCherry fusion gene that was unaffected by Met treatment. (C-E) Retina from larva expressing NTR-mCherry (red) in bipolar cells. (F-H) Retina from larva expressing NTR-mCherry (red) showed loss of fluorescently labelled bipolar cells following Met treatment. (I-P) The same type of analysis as in (A-H) conducted on larvae produced by mating of UAS:nfsB-mCherry transgenic fish to Tg(Gal4-VP16;UAS:eGFP)^xfz43. (N-P) Significant degeneration and loss of labelled bipolar cells occurred in the retina of Met treated larvae. Labelling to the left indicate Met treatment (+) and no treatment (-). The crosses and different types of fluorescence are indicated at the top. Scale bar: 50 μm.

Expression Data

Genes:	EGFP mCherry
Fish:	c264Tg; ub3Tg c264Tg; ub43Tg
Condition:	chemical treatment: metronidazole
Anatomical Term:	retinal bipolar neuron
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	c264Tg; ub3Tg c264Tg; ub43Tg
Condition:	chemical treatment: metronidazole
Observed In:	retinal bipolar neuron
Stage:	Day 5

Phenotype Detail

Acknowledgments

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