FIGURE

Fig. 5

ID

ZDB-FIG-090923-14

Publication

Zhao et al., 2009 - Labelling and targeted ablation of specific bipolar cell types in the zebrafish retina

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Fig. 5

Axon terminal patterns within the adult retina define distinct types of bipolar cells. (A-C) Confocal images of a retinal cryosection, labelled with an anti-GFP antibody (green) and counterstained with DAPI (blue), from an adult Tg(Gal4-VP16;UAS:eGFP)^xfz3fish (6 months). Higher magnification images of the middle part in (A) are shown in (B, C). The image in (B) is the same as in (C) but without DAPI. Arrows in (B, C) indicate a bouton-like axon terminal structure in the OFF layer, which is present in one bipolar cell type but absent in the other (arrowheads). (D-F) Retinal cryosection from an adult Tg(Gal4-VP16;UAS:eGFP)^xfz43fish. The confocal images are arranged in the same way as shown for the other line. Arrows in (E, F) indicate a large bouton-like structure in the ON sublamina, which is present at the axon terminal of one bipolar cell type. A second type of bipolar cells shows terminal arborisation within the OFF sublamina (arrowheads). ON and OFF sublamina of the inner plexiform layer are indicated by bars in (C, F). Scale bars: 50 μm (A, D); 25 μm (B, C, E, F). Abbreviations: gcl, ganglion cell layer; inl, inner nuclear layer; ipl, inner plexiform layer; onl, outer nuclear layer; opl, outer plexiform layer.

Expression Data

Gene:	EGFP
Antibody:	Ab3-GFP
Fish:	ub3Tg ub43Tg
Anatomical Terms:	OFF-bipolar cell ON-bipolar cell retinal bipolar neuron
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Neurosci.