FIGURE

Fig. 3

ID

ZDB-FIG-090717-16

Publication

Lamont et al., 2009 - Antagonistic interactions among Plexins regulate the timing of intersegmental vessel formation

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Fig. 3

Angioblast sprouting is delayed in plxnB2^i10e11 and plxnB2^i10e11/sema3e^ATG morphants. (A–B) PlxnB2 expression analysis by in situ hybridization shows semi-ubiquitous expression with ventral enrichment in the region surrounding the axial vessels in wholemount (A) and cross section views (B). The dashed line in A indicates the level of the section in B. (C–D) White light images of control (C) and plxnB2^i10e11 morphant (D) at 24 hpf. (E–H) Confocal images of uninjected (E), plxnB2^i10e11 morphant (F), sema3e^ATG morphant (G), and plxnB2^i10e11/sema3e^ATG morphant (H) at 24 hpf on a Tg(kdr:GFP)^la116 genetic background. (I) Graphical representation of the mean number and standard deviation of angioblast sprouts observed in plxnB2^i10e11 morphants, sema3e^ATG morphants, and plxnB2^i10e11/sema3e^ATG morphants as compared to control embryos. Scale bar in A, C–H is 100 μm and 30 μm in B.

Expression Data

Gene:	plxnb2a.1
Fish:	WT
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg + MO1-plxnb2a.1 la116Tg + MO2-plxnb2a.1 la116Tg + MO1-plxnb2a.1 + MO1-sema3e
Knockdown Reagents:	MO1-plxnb2a.1 MO1-sema3e MO2-plxnb2a.1
Observed In:	axial vasculature intersegmental vessel sprouting angiogenesis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 331(2), Lamont, R.E., Lamont, E.J., and Childs, S.J., Antagonistic interactions among Plexins regulate the timing of intersegmental vessel formation, 199-209, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.