FIGURE

Fig. 2

ID

ZDB-FIG-090717-15

Publication

Lamont et al., 2009 - Antagonistic interactions among Plexins regulate the timing of intersegmental vessel formation

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Fig. 2

Intersegmental sprouting is delayed in sema3e^e3i3 morphants and is precocious in obd mutants. (A) Graphical representation of the mean number and standard deviation of angioblast sprouts observed in sema3e^e3i3 morphants and obd^fov1b mutants as compared to control embryos. Confocal (B, D, F) and corresponding white light images (C, E, G) of uninjected (B, C), sema3e^e3i3 morphants (D, E), and obd^fov1b mutants (F, G) at 24 hpf on a Tg(kdr:GFP)^la116 genetic background. Scale bar in B, D, F is 100 μm and C, E, G is 500 μm.

Expression Data

Gene:	GFP
Fish:	la116Tg la116Tg + MO2-sema3e plxnd1^{fov01b/fov01b}; la116Tg
Knockdown Reagent:	MO2-sema3e
Anatomical Term:	blood vessel endothelial cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	la116Tg + MO2-sema3e plxnd1^{fov01b/fov01b}; la116Tg
Knockdown Reagent:	MO2-sema3e
Observed In:	axial vasculature intersegmental vessel sprouting angiogenesis
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 331(2), Lamont, R.E., Lamont, E.J., and Childs, S.J., Antagonistic interactions among Plexins regulate the timing of intersegmental vessel formation, 199-209, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.