FIGURE

Fig. 3

ID

ZDB-FIG-090710-35

Publication

Lunt et al., 2009 - Zebrafish ift57, ift88, and ift172 intraflagellar transport mutants disrupt cilia but do not affect hedgehog signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Expression of Hh target genes is unchanged in IFT mutants. In situ hybridization was performed for Hh target genes at 24 hpf. Embryos were photographed and subsequently genotyped. Wild type (+/+) and mutant (-/-) embryos are shown in individual panels for ift57 (A, D, G, J, M), ift88 (B, E, H, K, N), and ift172 (C, F, I, L, O) genotypes. A-C: Expression of ptc-1 and (D-F) ptc-2 remained unchanged in IFT mutants. A3-F4: High-magnification images of wild type and mutant embryos. G-I: gli1 and (J-L) gli3 remains unchanged at 24 hpf in wild type and IFT mutant embryos. M-O: High-magnification images of nkx2.2 staining in the ventral midbrain and hindbrain in wild type and IFT mutant embryos revealed no differences in expression. A1-O2: All images show lateral view of embryos, with anterior to the left. P-S: Sections of the neural tube from wild type and mutant embryos following in situ hybridization with ptc-1 reveal no differences in the dorsal or ventral expression boundaries. Arrows indicate the dorsal and ventral limits of ptc-1 expression.

Expression Data

Genes:	gli1 gli3 nkx2.2a ptch1 ptch2
Fish:	WT ift57^{hi3417Tg/hi3417Tg} ift88^tz288/tz288 ift172^{hi2211Tg/hi2211Tg}
Anatomical Terms:	central nervous system hindbrain midbrain ventral region neural tube somite somite posterior-most region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.