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Fig. S5

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ZDB-FIG-090527-37

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Phelps et al., 2009 - A two-step model for colon adenoma initiation and progression caused by APC loss

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Fig. S5

RAC1, but Not Cdc42, Activity Is Necessary for Nuclear Localization of β-Catenin in SW-480 Cells
(A) SW-480 cells were transfected with a RAC1-directed siRNA. Whole cell lysate were harvested and subjected to western blot analysis for total RAC1 and vinculin (top). Subcellular fractionation was performed on cells treated as above and the nuclear fraction was subjected to western blot analysis for β-catenin and histone H3 (bottom). (B) Sw-480 cells were transfected myc-tagged dominant negative Cdc42 construct. Whole cell lysate was collected and subjected to western blot analysis for myc and vinculin. Also, a Cdc42 activity assay was performed. (C) SW-480 cells were transfected with a RAC1-directed siRNA construct. Two days after transfection, the cells were fixed and stained for β- catenin (green) or ToPro3 (magenta) or whole cell lysates were harvested and subjected to western blot analysis for total RAC1 (top) or vinculin (bottom). All images are representative of at least three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 137(4), Phelps, R.A., Chidester, S., Dehghanizadeh, S., Phelps, J., Sandoval, I.T., Rai, K., Broadbent, T., Sarkar, S., Burt, R.W., and Jones, D.A., A two-step model for colon adenoma initiation and progression caused by APC loss, 623-634, Copyright (2009) with permission from Elsevier. Full text @ Cell