FIGURE

Fig. 3

ID

ZDB-FIG-090519-9

Publication

Kitaguchi et al., 2009 - Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis

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Fig. 3

Differential requirement of myeloid-expressed transcription factor on the expression of c/ebp1, pu.1 and runx1. Whole-mount in situ hybridization with probes shown at bottom right of each panel. Injected morpholinos are indicated at top right. All embryos are around 24 hpf and lateral view; anterior is left. (A–D) lyz expression was inhibited in pu.1 MO-, runx1 MO- and c/ebp1 MO-injected embryos. (E–G) The expression of c/ebp1 in myeloid cells was suppressed in pu.1 morphants, but not in runx1 morphants. (H–J) The runx1 expression in myeloid cells (red arrowheads; H and J), but not in the ICM and Rohon-beard cells, was inhibited in pu.1 morphants, while injection of c/ebp1 MO did not affect runx1 expression. (K–M) pu.1 expression in myeloid cells was comparably detected in uninjected-, runx1 MO-injected and c/ebp1 MO-injected embryos.

Expression Data

Genes:	cebp1 lyz runx1 spi1b
Fish:	WT WT + MO1-spi1b WT + MO3-cebp1 WT + MO4-runx1
Knockdown Reagents:	MO1-spi1b MO3-cebp1 MO4-runx1
Anatomical Terms:	intermediate cell mass of mesoderm myeloid cell Rohon-Beard neuron
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.