FIGURE

Fig. 5

ID

ZDB-FIG-090519-11

Publication

Kitaguchi et al., 2009 - Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis

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Fig. 5

Establishment of the lyz promoter-EGFP transgenic zebrafish, Tg(lyz:EGFP)^ko02. All pictures show lateral view; anterior is left. (A, B and F–I) Fluorescent images. A stable transgenic fish line Tg(lyz:EGFP)^ko02 expressing EGFP driven by the 2.4 kb lyz promoter. (A and B) EGFP-positive cells in Tg(lyz:EGFP)^ko02 at 26 hpf (A) or 3 dpf (B). (C–E) Two-color in situ hybridization with probes of EGFP and lyz: EGFP expression stained by FITC (green) completely overlapped endogenous lyz expression stained by FastRed (red). (F–I) Effect of morpholinos (runx1 MO, pu.1 MO and c/ebp1 MO) and mRNAs (runx1 + pu.1 + c/ebp1) on EGFP expression in Tg(lyz:EGFP)^ko02. Injected mRNAs or morpholinos are indicated at top right. The EGFP expression in Tg(lyz:EGFP)^ko02 was strongly suppressed in runx1 MO (0.5 ng)-injected, pu.1 MO (10 ng)-injected, or c/ebp1 MO (10 ng)-injected embryo compared to uninjected embryos (A). In contrast, the number of EGFP-positive cells increased in the embryos injected with mRNA (runx1 [100 pg] + c/ebp1 [100 pg] + pu.1 [10 pg]) in Tg(lyz:EGFP)^ko02.

Expression Data

Gene:	EGFP
Fish:	ko02Tg ko02Tg + MO1-spi1b ko02Tg + MO3-cebp1 ko02Tg + MO4-runx1
Knockdown Reagents:	MO1-spi1b MO3-cebp1 MO4-runx1
Anatomical Term:	myeloid cell
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 126(5-6), Kitaguchi, T., Kawakami, K., and Kawahara, A., Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis, 314-323, Copyright (2009) with permission from Elsevier. Full text @ Mech. Dev.