FIGURE

Fig. 5

ID

ZDB-FIG-090504-5

Publication

Serluca et al., 2009 - Mutations in zebrafish leucine-rich repeat-containing six-like affect cilia motility and result in pronephric cysts, but have variable effects on left-right patterning

Other Figures

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Fig. 5

Pronephric cyst phenotypes in seahorse mutants and sea MO-injected embryos. (A-F) At 2 dpf, the glomerular region (asterisks) appears normal in siblings (A) and sea^fa20r mutants (B), and slightly dilated in sea MO-injected embryos (C). In the same embryos, the medial tubules (arrows) were significantly dilated both in sea^fa20r mutants (E) and sea MO-injected embryos (F), compared with siblings (D). (G-L) At 2.5 dpf, the glomeruli (asterisks) in both the sea^fa20r mutants (H) and sea MO-injected embryos (I) were dilated compared with siblings (G). These same embryos have dilations in the medial tubules (arrows) in sea^fa20r mutants (K) and sea MO-injected embryos (L) compared with siblings (J). (M-O) At 3 dpf, siblings (M) display basolateral localization of Na⁺/K⁺-ATPase (red) in the pronephric epithelium (arrows; white arrowheads indicate lateral staining), whereas the sea mutants (N,O) display altered localization of Na⁺/K⁺-ATPase diffusely at apical (yellow arrowheads) and lateral membranes (white arrowheads).

Expression Data

Antibody:	Ab1-atp1a1
Fish:	WT dnaaf11^fa20r/fa20r WT + MO2-dnaaf11
Knockdown Reagent:	MO2-dnaaf11
Anatomical Terms:	epithelial cell apical plasma membrane epithelial cell basolateral plasma membrane epithelial cell lateral plasma membrane pronephros epithelium
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnaaf11^fa20r/fa20r WT + MO2-dnaaf11
Knockdown Reagent:	MO2-dnaaf11
Observed In:	establishment of epithelial cell apical/basal polarity pronephric glomerulus pronephric proximal straight tubule
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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