FIGURE

Fig. 3

ID

ZDB-FIG-090504-3

Publication

Serluca et al., 2009 - Mutations in zebrafish leucine-rich repeat-containing six-like affect cilia motility and result in pronephric cysts, but have variable effects on left-right patterning

Other Figures

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Fig. 3

seahorse mRNA is expressed in tissues that possess motile cilia. (A) RT-PCR of sea mRNA (503 bp) in cDNA libraries at the one- to two-cell, 256-cell, 1024-cell, sphere and 18-somite stages. sea mRNA was maternally expressed as zygotic transcription initiates after the 1024-cell stage. (B-J) Expression of sea was detected by RNA in situ hybridization in dorsal forerunner cells at 90% epiboly (B), in KV at 3 somites (C), and in the floor plate at 22 somites (D, arrowheads), 24 hpf (E,F arrowheads; G, bracket) and 48 hpf (J, arrowhead). Expression was detected in the pronephric tubules at 22 somites (D, arrows), 24 hpf (E,F, arrows), 36 hpf (H, arrow) and at 48 hpf (I,J, arrow). Open arrows in G and I indicate sea expression in the chordoneural hinge. Double-headed arrows in D,F and G span the width of the notochord, sea mRNA is not detected. Inset in F is a higher magnification image of the patchy pronephric expression that resembles expression of genes in multiciliated cells (Liu et al., 2007; Ma and Jiang, 2007).

Expression Data

Gene:	dnaaf11
Fish:	WT
Anatomical Terms:	chordo neural hinge floor plate forerunner cell group Kupffer's vesicle pronephric tubule pronephros whole organism
Stage Range:	1-cell to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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