Fig. 9

Apoptosis or caspase activity is partially responsible for the recruitment of neutrophils to the trunk of fad24^hi1019 mutant larvae. A,A′: TUNEL staining in the trunk of WT (A) and fad24^hi1019 mutants (A′). B: TUNEL staining in fad24^hi1019 mutant coimmunolabeled with MPO. C,C′: Acridine orange staining in the trunk of WT (C) and fad24^hi1019 mutant (C′) larvae. D,D′: Acridine orange staining in fad24^hi1019 larvae can be blocked by the addition of the pan-caspase inhibitor zVD.fmk (D′) to the larvae water but not by addition of the vehicle control, DMSO (D). E,E′: MPO immunolabeling of DMSO- (E) and zVD.fmk- (E′) treated fad24^hi1019 larvae. F: Quantification of neutrophils found in the body of fad24^hi1019 larvae when treated with zVD.fmk relative to the vehicle control, DMSO (set to 100%). Average of three replicates analyzed by a paired Student's t-test at a 95% confidence level. *P < 0.05, error bar is standard error of the mean. Lateral view with anterior to the left at 3 dpf. Scale bar = 200 μm. Representative results from three experiments with greater than 10 larvae per condition per experiment.