Fad24hi1019 mutants exhibit defects in lipid metabolism. A, A′: Oil red O staining in a PTU-treated WT (A) and fad24hi1019 (A′) larvae. Arrows, areas of staining in the head and heart; arrowheads, areas of staining in the vasculature that are absent in the fad24hi1019 mutant. B, C: Oil red O staining in the body of control (B) or zfad24 init. MO- (C) injected larvae. h, heart; isv, intersegmental vessel; da, dorsal aorta; dlav, dorsal longitudinal anastomotic vessel. Lateral view, anterior to the left at 3 dpf. Scale bar = 200 μm. Representative results from three experiments with greater than 10 larvae per condition per experiment.
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