Fig. 2

Injection of fad24 MO into WT embryos phenocopies the fad24^hi1019 leukocyte infiltration phenotype. Shown is the CHT and surrounding tissues of sudan black stained WT (A) and fad24^hi1019 (A′) larvae or control MO- (B), zfad24 init. MO- (C), or zfad24 ex17 MO- (D) injected larvae. Lateral view, anterior to the left at 3 dpf. Scale bar = 200 μm. E: Schematic of fad24 exon 16, 17, and 18 with the target site of the zfad24 ex17 MO labeled. Arrows, locations of primers used for RT-PCR of total RNA isolated from single embryos at 24 hr post-injection of either uninjected (lane 1), standard control (lane 2), or zfad24 ex17 (lanes 3 and 4) MO. F: Quantification of neutrophil location (either in the CHT or in the body) seen in WT and fad24^hi1019 larvae or control MO-, zfad24 init. MO-, or ex17 MO-injected larvae analyzed by one-way ANOVA at a 95% confidence level using Dunnett's multiple comparison post-test. *P < 0.05. Results reported as average ± SEM.