FIGURE

Fig. 4

ID

ZDB-FIG-081104-27

Publication

Laue et al., 2008 - Restriction of retinoic acid activity by Cyp26b1 is required for proper timing and patterning of osteogenesis during zebrafish development

Other Figures

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Fig. 4

cyp26b1 mutants and morphants display deficiencies in midline cartilages of the neurocranium and visceral skeleton. All panels show ventral views of zebrafish head regions. (A-D) col2a1 in situ hybridization at indicated stages. (E-N) Alcian Blue stainings of cartilaginous craniofacial elements at 120 hpf. Pharyngeal arches are numbered (1, mandibulare; 2, hyoid; 3-7, branchial/gill arches 1-5). (E-G) Overviews of visceral skeleton. (H-K) Magnified views of ceratohyals (H,I) or pharyngeal arches 4-6 (J,K). Arrows in H,I point to ceratohyal (ch) attachment in midline. (L-N) Flat-mounts of neurocranium, revealing the absence of medial ethmoid (e) and anterior basicranial commissure (abc) in mutant and morphant. anc, chondrocytes of anterior neurocranium; bb, basibranchial; bh, basihyal; cb, ceratobranchials; m, Meckel's cartilage; n, notochord; pq, palatoquadrate; t, trabeculae cranii.

Expression Data

Gene:	col2a1a
Fish:	WT cyp26b1^{ti230g/ti230g}
Anatomical Terms:	neurocranial trabecula chondrocyte neurocranium chondrocyte
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cyp26b1^{ti230g/ti230g} WT + MO5-cyp26b1
Knockdown Reagent:	MO5-cyp26b1
Observed In:	basibranchial chondrocranium cartilage embryonic cranial skeleton morphogenesis ethmoid cartilage parachordal cartilage
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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