FIGURE

Fig. 2

ID

ZDB-FIG-080925-2

Publication

Del Bene et al., 2008 - Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient

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Fig. 2

mok^s309 Retinas Have Increased RGCs and Decreased Bipolar and Müller Glia Cells Due to Premature Neurogenesis

(A–H) Horizontal 5 dpf retina sections. GFP expression under the control of atoh7 (A and E) and Brn3c (Pou4f3) (B and F) promoters reveals an increase in RGCs in mok^s309 (E and F) as compared to the wild-type (A and B), with some RGCs ectopically located outside the GCL (yellow arrowheads in [E] and [F]). The Müller glia marker GS (C and G) and the bipolar cell marker PKCα (D and H) show fewer immunoreactive cell bodies (yellow arrowheads) in mok^s309 (G and H) as compared to wild-type (C and D).

(I–P) Horizontal 50 hpf retina sections stained for IdU (green, injected at 26 hpf), BrdU (red, injected at 38 hpf), and DAPI (blue). mok^s309 retinas (M–P) show more IdU-positive and BrdU-negative cells than wild-type, indicating that a larger number of progenitors had exited the cell cycle between 26 and 38 hpf.

Scale bars, 100 μm.

Expression Data

Gene:	GFP
Antibodies:	Ab1-BrdU/IdU Ab1-glul Ab1-prkca Ab2-BrdU
Fish:	TL dctn1a^s309/s309 (TL) rw021Tg s356tTg (TL) dctn1a^s309/s309; rw021Tg (TL) dctn1a^s309/s309; s356tTg (TL)
Condition:	chemical treatment: 5-bromo-2'-deoxyuridine
Anatomical Terms:	retina retina retinal bipolar neuron retinal ganglion cell layer retinal ganglion cell retinal inner nuclear layer Muller cell
Stage Range:	Long-pec to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dctn1a^s309/s309 (TL) dctn1a^s309/s309; rw021Tg (TL) dctn1a^s309/s309; s356tTg (TL)
Condition:	chemical treatment: 5-bromo-2'-deoxyuridine
Observed In:	retina layer formation retinal ganglion cell retinal ganglion cell layer retinal ganglion cell
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 134(6), Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H., Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient, 1055-1065, Copyright (2008) with permission from Elsevier. Full text @ Cell