FIGURE

Fig. 7

ID

ZDB-FIG-080828-12

Publication

Dee et al., 2008 - Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm

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Fig. 7

Sox3 knock-down does not affect rostrocaudal patterning of the CNS. Control (A–E) and sox3 morphant (F–J) embryos were stained for the expression of positional markers of the developing brain and spinal cord. Expression of positional markers is maintained in morphant embryos despite severe disruption of CNS tissue. Markers used are emx1 (A, F, 42/49 embryos), otx2 (B, G, 10/10), pax2a (arrows indicate midbrain/hindbrain boundary; C, H, 19/22), krox20 (D, I, 14/14) and hoxb1b (arrows indicate rostral limit of CNS expression; E, J, 23/23). All embryos are aged 24 hpf and dorsal view except for panels E and J which are lateral view.

Expression Data

Genes:	egr2b emx3 hoxb1b otx2b pax2a
Fish:	WT WT + MO1-sox3 + MO2-sox3
Knockdown Reagents:	MO1-sox3 MO2-sox3
Anatomical Terms:	forebrain midbrain midbrain hindbrain boundary rhombomere 3 rhombomere 5 spinal cord telencephalon
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-sox3 + MO2-sox3
Knockdown Reagents:	MO1-sox3 MO2-sox3
Observed In:	central nervous system development rostrocaudal neural tube patterning spinal cord anterior/posterior patterning whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 320(1), Dee, C.T., Hirst, C.S., Shih, Y.H., Tripathi, V.B., Patient, R.K., and Scotting, P.J., Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm, 289-301, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.