FIGURE

Fig. 9

ID

ZDB-FIG-080828-14

Publication

Dee et al., 2008 - Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm

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Fig. 9

Sox3 knock-down causes abnormal development of the ear and cranial placodes. All embryos are aged 24 hpf. Otic vesicles of control (A, arrow indicates otolith) and sox3 morphant (B–D) embryos showing reduced (B, 25/49 embryos), split (C, 10/49), or absent vesicles (D, 14/49). (E–H) In situ hybridisation of control (E) and sox3 morphant (F–H) embryos viewed dorsally showing otic vesicles marked by the expression of pax2a again showing reduced (F), split (arrows, G) or absent (H) vesicles. (I, J) Lateral view showing placodal expression of ngn1 in control (I) and sox3 morphant embryos (J, 27/42). (K, L) Lateral view showing expression of phox2a in the epibranchial placodes of control (K) and sox3 morphant (L, 37/51) embryos. Expression of both ngn1 and phox2a is severely disrupted in the placodes of morphant embryos (J, L). ad, anterodorsal lateral line; av, anteroventral lateral line; f, facial; o/g, octaval/statoacoustic/glossopharyngeal.

Expression Data

Genes:	neurog1 pax2a phox2a
Fish:	WT WT + MO1-sox3 + MO2-sox3
Knockdown Reagents:	MO1-sox3 MO2-sox3
Anatomical Terms:	anterior lateral line placode epibranchial placode facial placode glossopharyngeal placode otic vesicle
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-sox3 + MO2-sox3
Knockdown Reagents:	MO1-sox3 MO2-sox3
Observed In:	neurogenic placode neuron differentiation otic vesicle
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 320(1), Dee, C.T., Hirst, C.S., Shih, Y.H., Tripathi, V.B., Patient, R.K., and Scotting, P.J., Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm, 289-301, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.