FIGURE

Fig. 3

ID

ZDB-FIG-080729-16

Publication

Sultana et al., 2008 - Zebrafish early cardiac connexin, Cx36.7/Ecx, regulates myofibril orientation and heart morphogenesis by establishing Nkx2.5 expression

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Molecular characterization of ftk gene. (A) Chromosome map of the ftk locus on LG 7 between markers z1206 and z14888. An enlarged map (brown line) and overlapping BAC clones (blue lines) are shown below. (B) Missense mutation in the zebrafish cx36.7 gene of the ftk mutant. (C and D) Rescue of the ftk phenotype by cx36.7 mRNA injection. Embryos obtained from crosses of ftk heterozygotes and injected with cx36.7 mRNA (30 pg) did not show the ftk phenotype at 36 hpf (C). (Inset) Sibling uninjected ftk mutant at 28 hpf, which displays the growing pericardiac edema (arrow). Genotyping of mRNA-injected embryos by RFLP analysis confirmed that two of eight embryos were genotypically ftk mutants (res1, res2) (D). (E) Whole-mount in situ hybridization analysis of cx36.7 expression during heart development. (Insets) Orientation of embryos.

Expression Data

Gene:	gjd6
Fish:	WT
Anatomical Terms:	heart heart primordium primitive heart tube
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gjd6^jk65/jk65 (TL)
Observed In:	pericardium
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA