FIGURE

Fig. 2

ID

ZDB-FIG-080729-15

Publication

Sultana et al., 2008 - Zebrafish early cardiac connexin, Cx36.7/Ecx, regulates myofibril orientation and heart morphogenesis by establishing Nkx2.5 expression

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

ftk mutation primarily affected the myofibril organization and nkx2.5 expression. (A–D) Myofibril organization examined by TEM analysis. In wild-type embryos at 58 hpf (A and B), myofibrils assemble as thick parallel bundles with orientation toward the longitudinal direction of the cardiomyocyte (double-headed arrows). In contrast, myofibrils in the ftk heart (C and D) are oriented in irregular directions. Double-headed arrows and asterisks indicate the myofibril bundles sectioned parallel and perpendicular to the long axis of the cells, respectively. Arrowheads, Z-lines; arrows, cell–cell contact sites; m, mitochondria. (Scale bars: 500 nm.) (B and D Insets) Schematic drawings of myofibril organization. (E) Whole-mount in situ hybridization analysis of nkx2.5 expression. In wild-type zebrafish embryos (Upper) The expression is detected in the developing heart; however, it is not detectable in ftk mutants (Lower). Mutants at early stages were genotyped by RFLP analysis. (Insets) Orientations of embryos.

Expression Data

Gene:	nkx2.5
Fish:	WT gjd6^jk65/jk65 (TL)
Anatomical Terms:	heart heart primordium primitive heart tube
Stage Range:	14-19 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gjd6^jk65/jk65 (TL)
Observed In:	cardiac myofibril assembly myocardium myofibril
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA