Fig. S3

Expression of embryonic patterning marker genes in ERK1 and ERK2 morphants Expression of mRNA was detected by whole mount in situ hybridization. (A–F, J–L); animal pole view, dorsal to right, (G–I,M–O) dorsal view, animal pole to top, (P–G′) lateral view, dorsal to right, animal pole to top. (A–C, P–R) gata2, expressed in non-neural ectoderm, at 80% epiboly. (D–F, SU) otx2, expressed in ectoderm at, 90% epiboly. (G–I, V–X)) cyclops/ndr2 in axial mesoderm at 80–90% epiboly. (J–L) snail1a in paraxial mesoderm at 90% epiboly. (M–O) sox17 in endoderm and forerunner cells at 80% epiboly. (Y–A′) notail in margin an axial mesoderm at 90% epiboly. (B′–D′) chordin in presumptive dorsal shield. (E′–G′) goosecoid in presumptive dorsal shield. Arrowheads mark the different boundaries of the different marker gene expression domains; the dotted line in P–X marks the boundary of epiboly progression.