FIGURE

Fig. 6

ID

ZDB-FIG-080701-27

Publication

McFarland et al., 2008 - Hh and Wnt signaling regulate formation of olig2(+) neurons in the zebrafish cerebellum

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Fig. 6

Wnt signaling is necessary for cerebellar olig2 expression. All embryos shown are 48 hpf. Anterior is to the left in all panels, the first column depicts a lateral whole mount view, the second column a dorsal view and the third a lateral view of the spinal cord. Black arrows mark cerebellum, brackets mark ventral spinal cord and ventral diencephalon is labeled (di). (A–C) Wild-type olig2 expression. (D–F) Df(LG01:lef1)^x8 (X8) mutant embryos expressed olig2 in spinal cord but not cerebellum. (G–I) Df(LG01:lef1)^x8 mutant embryos treated with CA at 6 hpf expressed olig2 in ventral diencephalon but not cerebellum or spinal cord. (J–L) hsDkk1GFP embryos heat shocked at 30 hpf expressed olig2 in ventral diencephalon and spinal cord but not cerebellum. (M–O) hsDkk1GFP embryos treated with CA at 6 hpf expressed olig2 in ventral diencephalon but not spinal cord or cerebellum. Scale bars represent 20 μm in all panels.

Expression Data

Gene:	olig2
Fish:	WT w32Tg/w32Tg Df(Chr01:lef1,msxb)x8/x8
Conditions:	chemical treatment: Cyclopamine chemical treatment: Cyclopamine, heat shock heat shock
Anatomical Terms:	cerebellum diencephalon spinal cord
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	w32Tg/w32Tg Df(Chr01:lef1,msxb)x8/x8
Conditions:	chemical treatment: Cyclopamine chemical treatment: Cyclopamine, heat shock heat shock
Observed In:	cerebellum spinal cord
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 318(1), McFarland, K.A., Topczewska, J.M., Weidinger, G., Dorsky, R.I., and Appel, B., Hh and Wnt signaling regulate formation of olig2(+) neurons in the zebrafish cerebellum, 162-171, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.