FIGURE

Fig. 6

ID

ZDB-FIG-080527-5

Publication

Kawahara et al., 2005 - Zebrafish GADD45{beta} genes are involved in somite segmentation

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Fig. 6

Biochemical activities of zebrafish GADD45β1. Indicated expression constructs (4 μg total: HA-GADD45β1, 2 μg; FLAG-MTK1-N, 2 μg; MTK1, 2 μg; pCS2 vector, 2 μg) were transfected into human 293T cells. Cell lysates were prepared after 24 h. (A) Cell lysates were immunoprecipitated with anti-FLAG gel and analyzed by Western blotting using anti-HA antibody (Top). Whole-cell lysates were used as controls for expression levels. (B) Phosphorylation state of p38 in the transfected cells. Cell lysates were analyzed by Western blotting using antibodies recognizing phospho-p38 and total p38. (C) Live embryos at the 10-somite stage. Synthetic RNA (human MTK1, 300 pg + EGFP, 300 pg) was injected into one blastomere of two-cell-stage embryos, leading to severe somite defects in the injection side.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA