Expression patterns of zebrafish gadd45β genes. Whole-mount in situ hybridization with probes for gadd45β1 (A–I) and gadd45β2 (J–R) is shown. Stages are indicated at the lower left of each micrograph. S, somite; hpf, hours postfertilization. Lateral view, dorsal is right (A and J) and anterior is left (B–D, F, H, I, L, N, and P–S). Dorsal view, anterior is left (E and O) and anterior is up (U). Anterior view, dorsal is right (K). Posterior view, dorsal is up (G, M, and T). Expression of gadd45β1 in the YSL at the shield stage is shown in A (green arrowhead). gadd45β2 expression in the shield and involuting mesoderm is shown in J–L (red arrowhead). At bud stage, one bilateral stripe arises for both gadd45β1 and gadd45β2 at the anterior edge of presomitic mesoderm (C, L, and M; black arrowhead); weak expression of gadd45β2 is observed in the tail-bud mesoderm (L and M; yellow arrowhead). Expression in anterior presomitic mesoderm is maintained throughout somitogenesis (C–G and L–P; black arrowhead). Weak expression of gadd45β2, but not gadd45β1, is maintained in the somites. At 24 hpf, gadd45β1 expression is newly detected in the lens of the eye (H, blue arrowhead). (S) Whole-mount in situ hybridization with both gadd45β1 and gadd45β2 probes shows coexpression in the anterior PSM (black arrowhead). (T) Double in situ hybridization shows that gadd45β1 (purple and black arrowhead) is located just anterior of the second her1 expression domain (red and red asterisks) in the anterior PSM. (U) Double in situ hybridization shows that gadd45β1 is positioned posterior to the MyoD expression domain (red and red bracket), leaving a gap between the domains.
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