Fig. 7

This figure illustrates that knockdown of Gadd45 expression does not affect general mesodermal markers in the embryo. The overexpression of MTK1/MEKK4 leads to reduced and disorganized expression of somite pattern genes. (A–L) Effect of gadd45β MO on marker gene expression. MOs were injected into one- or two-cell-stage embryos, as shown in Fig. 3. Lateral view, anterior is left (A–I). Dorsal view, anterior is up (J–L). Expression of tbx6, tbx24, wnt3l, and ntl was not affected in the double gadd45β-MO-injected embryos. (M–R) Effect of MTK1/MEKK4 RNA overexpression on marker gene expression. Posterior view, anterior is up (M–P). Dorsal view, anterior is up (Q and R). Expression of mesp-a, her1, and MyoD was partly suppressed, and the pattern was disorganized in MTK1 RNA-injected embryos.