hdac3 is required for early liver formation. WISH with three hepatoblast markers hhex (A–D), prox1 (E–H) and foxa3 (I–L) was analyzed in hdac3 morphants. At 28–30 hpf, hhex expression in liver (white arrow) was greatly reduced in hdac3 morphants (A vs. B). It remained reduced at 48 hpf (C vs. D) whereas its expression in pancreatic islet (black arrow) was less affected. prox1 expression was reduced in liver region at 28–30 hpf (white arrow, E vs. F) and at 48 hpf (G vs. H), while its expression in mesoderm (E and F, green arrow) and pancreas (G and H, black arrow) is less affected. At 28–30 hpf, liver budding (shown as anterior endoderm thickening) was absent in hdac3 MO (I vs. J, white arrow) as judged by foxa3 expression and a reduced liver region was observed at 48 hpf (K, L). foxa3 expression in pancreas region (I, J, black arrow and Pn in K, L) was less affected in both stages. Swim bladder was absent at 48 hpf in hdac3 MO (K vs. L). Lv: liver; Pn: pancreas; in: intestine; Sb: swim bladder. Scale bar represents 100 μm in panels A–F; 50 μm in panels G–L.
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