Fig. 7

Temperature down-shift experiments show that anastomosis can be delayed with no apparent consequence to the plexus formation. While wild-type fish (A–C) always complete anastomosis successfully and develop normal plexuses in these experiments, most regenerating vessels in reg6 mutants (D–F) had failed to complete formation of anastomotic bridges (D) at the time of shift (26 hpa) (yellow arrowheads point out the absence of anastomotic bridge at amputation plane; also see Table 1). (E) Although the vessels developed small bulbs at the distal ends (yellow arrows), they were able to form anastomotic bridges at a more distal position (white arrow) 12 hps. (F) These vessels developed normal plexuses within 2 days after shift to the permissive temperature. hps, h postshift. Red arrow, amputation plane; yellow arrow, swollen vessels; double-headed arrow, plexus. Scale bar, 100 μm.