Fig. 5
Neuronal differentiation and lamination of the retina in Fgf19 MO1-injected embryos. (a, b) The expression of neurod in control and Fgf19 MO1-injected embryos at 45 hpf. The expression of neurod in the outer retina was not affected in Fgf19 MO1-injected embryos. Lateral views with anterior to the left and dorsal to the top. (c, d) The expression of isl1 in control and Fgf19 MO1-injected embryos at 48 hpf. The expression of isl1 in the retina was not affected in Fgf19 MO1-injected embryos. Lateral views with anterior to the left and dorsal to the top. (e, f) The detection of HuC/D protein in control and Fgf19 MO1-injected embryos at 5 dpf. HuC/D was normally detected in the amacrine and ganglion cells of Fgf19 MO1-injected embryos. (g, h) Transverse eye sections of control and Fgf19 MO1-injected embryos at 3 dpf. The sections were stained with toluidine blue. The control retina showed a characteristic stratification into three nuclear layers and two plexiform layers. In Fgf19 MO1-injected embryos, the stratification of retinal layers occurred in the dorsal region, although the eye exhibited a reduction in size. However, the lamination of the ventral retina was abnormal in Fgf19 MO1-injected embryos. |
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| Stage: | High-pec |
Reprinted from Developmental Biology, 313(2), Nakayama, Y., Miyake, A., Nakagawa, Y., Mido, T., Yoshikawa, M., Konishi, M., and Itoh, N., Fgf19 is required for zebrafish lens and retina development, 752-766, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.