Fig. S3

Further repression of Foxd3 in hdac1^col-/-;foxd3^zdf10+/- and hdac1^col-/-;foxd3^zdf10+/+ mutants increases the rate of rescue of melanogenesis. A-D: Side views of live images of hdac1^col embryos at 3.5 dpf obtained from hdac1^col+/-;foxd3^zdf10+/- heterozygous mutant incrosses in which either Foxd3 is further reduced with 0.477 ng of foxd3 morpholino (mo) or are uninjected controls. A: At 3.5 dpf 10% of hdac1^col embryos obtained from a double mutant hdac1^col+/-;foxd3^zdf10+/- heterozygous incross have increased number of melanophores which migrate over the head, into the ventral stripe (v) in the tail and onto the yolk sac areas (y). The genotype of the rescued mutants was identified as hdac1^col-/-;foxd3^zdf10+/-. B: hdac1^col-/-;foxd3^zdf10-/- double homozygous mutants have fewer melanophores than hdac1^col-/- single mutants and most of the melanophores are present in the dorsal (d) stripe. C, D: Reducing Foxd3 via morpholino rescues the melanophore defect in hdac1^col-/-;foxd3^zdf10+/- and hdac1^col-/-;foxd3^zdf10+/+ mutants (62.5% of all hdac1^col mutants) but not in hdac1^col-/-;foxd3^zdf10-/- double homozygous mutants. A-D: hdac1^col-/-;foxd3^zdf10+/-, hdac1^col-/-;foxd3^zdf10+/+ and hdac1^col-/-;foxd3^zdf10-/- mutants have the same eye defect as hdac1^col-/- single mutants and knockdown of Foxd3 has no effect on the eye phenotype (arrows).