Fig. 4

Neural crest induction and migration of non melanogenic cells are largely unaffected in hdac1^col mutants. In situ hybridizations for tfap2a (A, E, F), sox9b (B), sox10 (C, G, H, O, P), snai1b (D), foxd3 (I, J) and ctn (K, L, M, N) between 3 somites (s) and 24 hpf. (A–D) Dorsal views of representative gene expression in 3 somite embryos. Neural crest induction is normal and there is no difference in the expression of tfap2a, sox9b, sox10 and snai1b between wild-type and hdac1^col mutants. (E–L) Lateral views of 15 somite embryos. At the 15 s stage there is no difference in the expression and number of cranial and trunk neural crest cells (arrowheads) expressing tfap2a (E, F), sox10 (G, H), foxd3 (I, J) and ctn (K, L) between wild-type and hdac1^col mutants. (M–P) Lateral views of 24 hpf embryos. Later by 24 hpf there is a slight reduction in the number of trunk neural crest cells expressing ctn and sox10 in hdac1^col mutants compared to wild-type. However, the overall migration of trunk neural crest cells in hdac1^col mutants is largely unaffected although slightly delayed (arrows, arrowheads).