Fig. 6

Repression of Foxd3 rescues melanogenesis in hdac1^col mutants. Images of live embryos at 3 dpf, (A–D) lateral views, (E–H) dorsal views. (A, B) There are fewer melanophores present in hdac1^col mutants at 3 dpf compared to wild-type embryos. Melanophores present in hdac1^col mutants fail to migrate and are present at the site of origin in the post otic region, in the dorsal (d) stripe and in the anterior ventral stripe. In wild-type embryos, melanophores migrate to form four stripes; dorsal (d), lateral (l), ventral (v) and yolk (y). (C, D) Partial knockdown of Foxd3 in hdac1^col mutants increases melanophore numbers and the melanophores migrate over the head (arrowhead), into the ventral stripe and over the yolk. (E, F) In control uninjected hdac1^col mutants the dorsal stripe (d) is usually 3–5 melanophores wide (arrowhead), in comparison the wild-type dorsal stripe is 2ipe and over the yolk. (E, F) In control uninjected hdac1^col mutants the dorsal stripe %–3 melanophores wide (arrowhead). (G, H) Partially reducing Foxd3 with a translation blocking morpholino (mo) in hdac1^col mutants increases the migration of melanophores into the ventral stripe and over the yolk, the resulting dorsal stripe in hdac1^col/foxd3 mo mutant/morphants is only 2–3 melanophores wide (arrowhead).