FIGURE

Fig. 4

ID

ZDB-FIG-080326-121

Publication

Bonner et al., 2008 - Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling

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Fig. 4

Tcf3 is required for proliferation but not for dorsal progenitor patterning. (A–D) Both tcf3a (A, B) and tcf3b (C, D) are expressed at higher levels in tcf7 morphants. (E) The mitotic index in dorsal, intermediate and ventral domains is significantly reduced in tcf3a/tcf3b double morphants. *p < 0.05 by T-test, error bars indicate ± SEM. (F, G) Expression of msxc and (H, I) pax3 is unaffected in tcf3a/tcf3b double morphants. (J, K) Expression of dbx2 is eliminated in tcf3a/tcf3b double morphants, but the intermediate progenitor domain is not shifted dorsally as indicated by iro3 expression (L, M). (A–D) 12 μm cross-sections are shown. (F–M) Lateral mounts are shown. Scale bars = 10 μm.

Expression Data

Genes:	dbx2 irx3a msx3 pax3a tcf7l1a tcf7l1b
Fish:	AB AB + MO1-tcf7 AB + MO2-tcf7l1a + MO3-tcf7l1b
Knockdown Reagents:	MO1-tcf7 MO2-tcf7l1a MO3-tcf7l1b
Anatomical Term:	spinal cord
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-tcf7l1a + MO3-tcf7l1b
Knockdown Reagents:	MO2-tcf7l1a MO3-tcf7l1b
Observed In:	cell population proliferation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 313(1), Bonner, J., Gribble, S.L., Veien, E.S., Nikolaus, O.B., Weidinger, G., and Dorsky, R.I., Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling, 398-407, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.