FIGURE

Fig. 5

ID

ZDB-FIG-080324-9

Publication

Smith et al., 2008 - Gene expression analysis on sections of zebrafish regenerating fins reveals limitations in the whole-mount in situ hybridization method

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Fig. 5

Comparison of the expression of lef1 in a 4-day fin regenerate using the two ISH methods. A: Whole-mount ISH with a lef1 probe on 4-dpa regenerates shows expression splitting into two domains in the distal part of each fin ray indicating an imminent bifurcation event. B: Upon cryo-sectioning of the fin regenerate shown in A, lef1 expression is observed in the basal layer of the epidermis and in the distal blastema. C: A similar expression pattern is observed when an ISH is performed on cryo-sections of 4-dpa regenerates. The arrow in A indicates the level of amputation. L, lepidotrichia; b, blastema; ble, basal layer of epidermis; s, scleroblasts. Scale bars = 100 mu;m (A), 50 μm (B,C).

Expression Data

Gene:	lef1
Fish:	WT
Condition:	amputation: caudal fin
Anatomical Terms:	basal regeneration epithelium blastema
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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