PUBLICATION
Gene expression analysis on sections of zebrafish regenerating fins reveals limitations in the whole-mount in situ hybridization method
- Authors
- Smith, A., Zhang, J., Guay, D., Quint, E., Johnson, A., and Akimenko, M.A.
- ID
- ZDB-PUB-080102-7
- Date
- 2008
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 237(2): 417-425 (Journal)
- Registered Authors
- Akimenko, Marie-Andree, Johnson, Alyce, Quint, Elizabeth
- Keywords
- in situ hybridization, zebrafish, fin regeneration, cryo-section
- MeSH Terms
-
- Animals
- Azo Compounds
- Cryopreservation
- Gene Expression Profiling/methods*
- Gene Expression Regulation, Developmental/physiology*
- In Situ Hybridization/methods*
- Regeneration/physiology*
- Tail/physiology*
- Zebrafish/physiology*
- PubMed
- 18163531 Full text @ Dev. Dyn.
Citation
Smith, A., Zhang, J., Guay, D., Quint, E., Johnson, A., and Akimenko, M.A. (2008) Gene expression analysis on sections of zebrafish regenerating fins reveals limitations in the whole-mount in situ hybridization method. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(2):417-425.
Abstract
The caudal fin of adult zebrafish is used to study the molecular mechanisms that govern regeneration processes. Most reports of gene expression in regenerating caudal fins rely on in situ hybridization (ISH) on whole-mount samples followed by sectioning of the samples. In such reports, expression is mostly confined to cells other than those located between the dense collagenous structures that are the actinotrichia and lepidotrichia. Here, we re-examined the expression of genes by performing ISH directly on cryo-sections of regenerates. We detected expression of some of these genes in cell types that appeared to be non-expressing when ISH was performed on whole-mount samples. These results demonstrate that ISH reagents have a limited capacity to penetrate between the regenerating skeletal matrices and suggest that ISH performed directly on fin sections is a preferable method to study gene expression in fin regenerates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping