JNK Is Involved in Dorsoventral Patterning (A) Expression patterns of marker genes in wild-type embryos or embryos injected with 300 pg of MKK4-ED mRNA, 10 ng of MKK4-MO, or both. Embryos for chd and eve1 are animal pole views with dorsal oriented toward the right; embryos for gsc are in dorsal views with animal pole to the top; and embryos for bmp2 and gata2 are in lateral views with dorsal to the right. (B) Expression patterns of marker genes in wild-type embryos or embryos injected with 300 pg of JNK mRNA, 10 ng of JNK-MO, or both. Experiments are performed as described in (A). (C and D) Statistical data for (A) and (B), respectively. (E) Percentages of embryos with expression changes of marker genes in shield stage caused by axin mRNA injection or coinjection of axin and MKK4-MO. Approximately 200 pg of axin mRNA was injected into one-cell embryos alone or together with 10 ng of MKK4-MO. In situ hybridization analysis of different marker genes in injected embryos was performed. Percentages of embryos with elevated, normal, or reduced expression levels of the marker genes were calculated and diagramed. (F) Ratios of dorsalized and ventralized embryos derived from the same experiments as those detailed for (E), except that JNK-MO was used instead of MKK4-MO.
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