Fig. 8

Effects of scl antisense morpholino injection on the interrenal tissue. (A–D) Lateral views of embryos injected with STD-MO (A, C) and sclMO (B, D), respectively, harvested at 51 hpf, and subject to either DAF staining (A, B) or no staining (C, D) prior to Nomarski photomicroscopy. Blood cells are present in the anterior portion (A) and tail vessels (C) of STD-MO-injected embryos, but not detected in the scl morphants (B, D). (E–G) Single confocal sections showing the dorsal views of interrenal tissues as stained by 3β-Hsd activity assay (E), and neighboring ECs as labeled by green fluorescence (F), of 53 hpf Tg(fli1:EGFP)^Y1 embryos injected with sclMOs. The merged images of 3β-Hsd activity and GFP are shown in panel G. Anterior is oriented to the left in panels A–D and to the top in panels E–G. The central convergence of interrenal tissues is not affected in scl morphants where blood cells are absent. Black arrows indicate blood cells, and red arrows indicate interrenal tissues.