FIGURE

Fig. 9

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ZDB-FIG-060929-13
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Liu et al., 2006 - Endothelium is required for the promotion of interrenal morphogenetic movement during early zebrafish development
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Fig. 9

Effects of forced expression of SCL on the trunk endothelium and interrenal tissue in Tg(flk1:EGFP)s843;clom39 mutants. Single confocal sections showing the interrenal tissues as detected by 3β-Hsd activity staining (Left panel: A, D, G), and the neighboring ECs as revealed by green fluorescence from Tg(flk1:EGFP)s843 background (B, E, H), of 78 hpf homozygous clom39 mutants (D–I) and their wild-type siblings (A–C), injected either with pCS2+ plasmid control (cmv; A–F) or pCS2+SCL (cmv-SCL; G–I). The merged images of 3β-Hsd activity staining and GFP are shown in the right panel (C, F, I). All panels are dorsal views with anterior oriented to the top. The trunk vasculature as well as interrenal phenotypes of wild-type siblings being injected with cmv-SCL were essentially not varying from those injected with cmv, and are hence not shown. The defective fusion of bilateral interrenal primordia in clom39 is alleviated while the trunk endothelium is partially rescued. DA, dorsal aorta; PCV, posterior cardinal vein. Red arrowheads indicate interrenal tissues. Scale bar, 50 μM.

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Reprinted from Developmental Biology, 297(1), Liu, Y.W., and Guo, L., Endothelium is required for the promotion of interrenal morphogenetic movement during early zebrafish development, 44-58, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.