Fig. 6

 Injection of stau1 and stau2 morpholinos causes loss of germ cells. (A–H) Expression of the germ cell specific gene, vasa, in 4 cell (A, B), shield stage (C, D), and 24 hpf (E–H) embryos injected with control morpholinos (A, C, E, G), Stau2MO1 (H), or Stau2 MO2 (B, D, F). GFP:nos1 expression in shield stage (I, J), 3-somite stage (K, L) and 24 hpf (M–P) embryos injected with control morpholinos (I–O), Stau2 MO1 (P), or Stau2 MO2 (J–N). Schematic diagrams in panels Q, R, and S show the position of morpholinos targeting exon junctions in stau1 (Q) or stau2 (R, S), with primer positions indicated. RT-PCRs show amplification of cyclinB control cDNA, stau1 (Q) or stau2 (R, S) products in control or morpholino-injected embryos. Histogram in panel T shows % embryos with GFP expression in PGCs. Scale bars, 50 μm in panels A, C, I, K and 100 μm in panels G, M, and insets.