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Fig. 5

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ZDB-FIG-070917-56

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Ramasamy et al., 2006 - Zebrafish Staufen1 and Staufen2 are required for the survival and migration of primordial germ cells

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Fig. 5

Stau1 and Stau2 peptides overexpression abolishes PGCs. (A–F) Expression of the germ cell specific gene, vasa, in 4 cell (A, B), shield stage (C, D), and 24 hpf (E, F) embryos injected with GST (A, C, E), Stau2 RBD5 (B, D, F); GFP:nos1 expression in shield stage (G, H), 3-somite stage (I, J), and 24 hpf (K, L) embryos injected with GST (G, I, K), Stau2 RBD5 (H, J, L), histogram in M shows % embryos with GFP expression in PGCs. In panels I–L, dotted boxes outline the areas shown at higher magnification in the insets. A–D, animal pole views; E–H and K, L, lateral views; I, J dorsal views. Scale bars, 50 μm for panels A, C, G, I and 100 μm for panels E, K, and insets.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 292(2), Ramasamy, S., Wang, H., Quach, H.N., and Sampath, K., Zebrafish Staufen1 and Staufen2 are required for the survival and migration of primordial germ cells, 393-406, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.