FIGURE

Fig. 4

ID

ZDB-FIG-060809-13

Publication

Nissen et al., 2006 - A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for Endothelin-1 expression

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Fig. 4

wdr68 activity is required for all 1^starch cartilage formation. A), C), E), G) Lateral views of Alcian blue stained animals at 4dpf. B), D), F), H) Ventral views of Alcian blue stained animals at 4dpf. In panels D, F, and H the posterior arches were removed to enhance clarity for observing the anterior arch defects. A), B) Wild type sibling. C), D) Representative 'strong' phenotype dys/dys animal similar to that shown flatmounted in figure 1 panel D with incomplete loss of M and PQ cartilages. The wdr68MO when injected into wild type animals yields a phenotype very similar to that shown here in panels C and D. E), F) Injecting dys/dys animals with antisense wdr68MO causes complete loss of the PQ, M and CH but does not substantially reduce the HM region. G), H) Representative edn1MO knockdown animal lacking the M and CH but retaining the PQ and HM regions.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dcaf7^{hi3812Tg/hi3812Tg} dcaf7^{hi3812Tg/hi3812Tg} + MO1-dcaf7 WT + MO1-dcaf7 WT + MO1-edn1
Knockdown Reagents:	MO1-dcaf7 MO1-edn1
Observed In:	ceratohyal cartilage Meckel's cartilage metapterygoid palatoquadrate cartilage symplectic
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.