FIGURE

Fig. 2

ID

ZDB-FIG-060809-11

Publication

Nissen et al., 2006 - A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for Endothelin-1 expression

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Fig. 2

The dys^hi3812mutant is a null. A) Diagram indicating the location of the dys^hi3812proviral insert in the first exon of the wdr68 gene. B) RT-PCR analysis of wild type siblings and dys/dys animals showing that wild type wdr68 transcripts are not detectable in the dys mutants indicating that the mutant is a null allele. C) RT-PCR analysis reveals a maternal supply of wdr68 transcripts. cDNA from wild type embryos at zero = unfertilized, 3 hpf, 6 hpf, 10 hpf, and 19 hpf. As negative control, edn1 transcripts are only detected after 10 hpf. Transcripts for the ribosomal gene rpL35 are present at all stages examined.

Expression Data

Genes:	dcaf7 edn1 rpl35
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	1-cell to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.