FIGURE

Fig. 2

ID

ZDB-FIG-060605-31

Publication

Hutchinson et al., 2006 - Islet1 and Islet2 have equivalent abilities to promote motoneuron formation and to specify motoneuron subtype identity

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Fig. 2

Islet1 is required for PMN formation. (A-C) 20 hpf embryos stained with islet2 riboprobe. Control embryos (A) express islet2 in RBs (dorsally located cells) and CaPs (ventrally located cells). islet1 MO-injected embryos (B) lack islet2 expression. islet1 MO-injected embryos co-injected with islet1 RNA (C) also lack most islet2 expression; one islet2-positive PMN is indicated by a black dot. (D-F) 28 hpf embryos stained with zn1 and znp1 Abs (green). Control embryos (D) have dorsally projecting MiP axons (arrows) and ventrally projecting CaP axons (arrowheads). islet1 MO-injected embryos (E) lack both MiP and CaP axons. Co-injection of islet1 MO and islet1 RNA (F) restored both MiP and CaP axons. Scale bar: 20 µm.

Expression Data

Gene:	isl2a
Antibodies:	zn-1 znp-1
Fish:	AB AB + MO2-isl1a + MO3-isl1a
Knockdown Reagents:	MO2-isl1a MO3-isl1a
Anatomical Terms:	CaP motoneuron MiP motor neuron primary motor neuron Rohon-Beard neuron
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-isl1a + MO3-isl1a
Knockdown Reagents:	MO2-isl1a MO3-isl1a
Observed In:	CaP motoneuron axon MiP motor neuron axon
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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