FIGURE

Fig. 2

ID

ZDB-FIG-060201-2

Publication

Hamade et al., 2006 - Retinoic acid activates myogenesis in vivo through Fgf8 signalling

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Fig. 2

Repression and activation, respectively, of the expression of myoD by DEAB and RA. Flat-mounted embryos, anterior is to the left. (A–B) Comparison of myoD expression in wild-type (A) and neckless (nls; B) siblings at the 12-somite stage. At this stage, myoD is expressed in the lateral aspect of somites (arrowhead) and two rows of adaxial cells (arrows) in wild-type embryos (A) and krox20 is expressed in rhombomeres 3 and 5 (asterisks). The shorter distance between rhombomere 5 and trunk mesoderm (myoD) has been used to identify nls embryos (brackets in panels A and B). Expression of myoD is reduced in nls embryos (B). (C) Closer view restricted to the trunk showing expression of myoD in 10-somite stage embryo. (D) In DEAB-treated embryos, myoD expression is reduced in somites, as well as in adaxial cells (white arrowhead). (E) In RA-treated embryos, myoD expression is up-regulated in somites and strongly induced in anterior psm (bracket). (F) Control at the 12-somite stage. (G–H) Incubation in retinal (Ral) produces the same phenotype as incubation in RA. Embryos incubated in DEAB and Ral are undistinguishable from control embryos demonstrating that Ral needs to be converted locally in RA by Raldhs to induce myoD expression. psm, presomitic mesoderm. Scale bars: in panel A, 100 μm for panels A–B; in panel C, 100 μm for panels C–H.

Expression Data

Genes:	egr2b myod1
Fish:	WT aldh1a2^i26/i26
Conditions:	chemical treatment by environment: 4-(diethylamino)benzaldehyde chemical treatment: all-trans-retinoic acid chemical treatment: all-trans-retinal chemical treatment: pharmaceutical
Anatomical Terms:	adaxial cell rhombomere 3 rhombomere 5 segmental plate somite
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 289(1), Hamade, A., Deries, M., Begemann, G., Bally-Cuif, L., Genet, C., Sabatier, F., Bonnieu, A., and Cousin, X., Retinoic acid activates myogenesis in vivo through Fgf8 signalling, 127-140, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.