Fig. 5

Knockdown of Ved in vent; vox mutants replicates the phenotype of wnt8; swr mutants. In situ hybridization for flh (A–D, Q–T), eve1 (E–H), tbx24 (I–L, W–X), myoD (M–P), and ved (U–V). Genotypes are indicated. A–H: shield stage, animal views, dorsal right. I–L, W–X: 70% epiboly, lateral views, dorsal right. M–V: bud stage, dorsal view (M–T) or lateral views, dorsal right (U–V). Insets M–T: vegetal views, dorsal up. Insets U–V: vegetal view, dorsal right. flh expression in vent; vox mutants is expanded ventrally but not in a robust fashion (arrow in panel B, inset in panel R). Injection of ved MO in vent; vox mutants results in a stronger expansion of flh (arrow in panel D, inset in panel T). The expression of the non-axial mesoderm markers eve1, tbx24, and myoD is strongly reduced in the vent/vox/ved triple knockdown (H, L, P). At bud stage, vent; vox mutants still display ved expression at the margin but not in the future epidermis (U, V). Injection of vent RNA in wnt8; swr double mutants does restore some tbx24 expression but does not rescue the phenotype to wild-type (W, X, compare to I).