Fig. 2

Gene expression analysis of hecate mutant embryos. Whole mount in situ hybridization of embryos shows a reduction in the expression of dorsal genes: nieuwkoid (nwk), at sphere [4 hpf; (a, b)]; goosecoid (gsc), at 50% epiboly [5.3 hpf; (c, d)]; as well as an expansion in the expression of ventral genes: even skipped 1 (eve), at 50% epiboly (e, f); bmp2, at 80% epiboly [7.3 hpf; (g, h)]; gata2, at 80% epiboly (i, j). Expression of the mesendodermal marker gene no tail at 50% epiboly (k, l) and the endodermal marker foxa2 (axial) at 80% epiboly (m, n) show no reduction in germ layer specific staining, although as expected the dorsal domains of expression of these genes are reduced. All panels are animal views with dorsal side to the right (when identifiable), except panels m, which is a dorsal view.