Fig. 6

Clustering behavior of cultured mesendodermal (hypoblast) and ectodermal (epiblast) progenitor cells from wild-type/cyc-overexpressing (hypoblast) and mz-oep (epiblast) embryos in the absence or presence of E-Cadherin. (A-D) Hypoblast cells from wild-type/cyc-overexpressing (A,C; red) and e-cadherin morphant/cyc-overexpressing (B,D; green) embryos, after plating out (A,B; t=0) and after 16 hours in culture (C,D; o/n). (E) Wild-type/cyc-overexpressing hypoblast cells labeled in red or green (`control/control′) after 16 hours in culture (o/n). (F) Wild-type/cyc-overexpressing hypoblast cells (labeled in red) mixed with e-cadherin morphant hypoblast cells (labeled in green) after 16 hours in culture (o/n). (G-L) Epiblast cells from mz-oep (G,I; red) and e-cadherin morphant mz-oep (H,J; green) embryos, after plating out (G,H; t=0) and after 16 hours in culture (I,J; o/n). (K) mz-oep epiblast cells labeled in red or green (`control/control′) after 16 hours in culture (o/n). (L) mz-oep epiblast cells (labeled in red) mixed with e-cadherin morphant mz-oep epiblast cells (labeled in green) after 16 hours in culture (o/n). (M-O) Wild-type/cyc-overexpressing hypoblast cells labeled in red mixed with mz-oep epiblast cells labeled in green, after plating out (M; t=0) and after 16 hours in culture (N,O; o/n); at 16 hours, mixed cultures are shown for control epiblast and hypoblast cells (N), and for epiblast and hypoblast cells injected with e-cadherin MO (O). Green, fluorescein; red, rhodamine.