CRISPR

CRISPR3-gata5

ID

ZDB-CRISPR-210322-1

Name

CRISPR3-gata5

Previous Names

None

Target

gata5 (1)

Sequence

5' - GGGCGCGAGTGTGTGAACTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hsc115	gata5
wcm10	gata5
wcm11	gata5
wcm8	gata5
wcm9	gata5

Expression

Gene expression in Wild Types + CRISPR3-gata5

No data available

Phenotype

Phenotype resulting from CRISPR3-gata5

No data available

Phenotype of all Fish created by or utilizing CRISPR3-gata5

Phenotype	Fish	Conditions	Figures
cardiac muscle progenitor cell migration to the midline involved in heart field formation decreased occurrence, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. 3. from Sam et al., 2020
heart tube split bilaterally, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. 4. from Sam et al., 2020
heart bifurcated, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. S1 from Sam et al., 2020
pericardium edematous, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. 1. , Fig. S1 from Sam et al., 2020
cardiac muscle cell myl7 expression decreased amount, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. 3. , Fig. 4. from Sam et al., 2020
heart primordium nkx2.5 expression decreased amount, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. 3. from Sam et al., 2020
cardiac muscle cell myl7 expression spatial pattern, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. 3. from Sam et al., 2020
whole organism dead, abnormal	gata5^wcm8/wcm8	standard conditions	text only from Sam et al., 2020
myocardial precursor mislocalised laterally, abnormal	gata5^wcm8/wcm8	standard conditions	Fig. 3. , Fig. 4. from Sam et al., 2020
heart tube decreased thickness, abnormal	gata5^wcm8/+; gata4^wcm6/wcm6	standard conditions	Fig. 4. from Sam et al., 2020
cardiac muscle cell myl7 expression decreased amount, abnormal	gata5^wcm8/wcm8; gata4^wcm6/+	standard conditions	Fig. 4. from Sam et al., 2020
heart tube morphology, ameliorated	gata5^wcm8/wcm8; gata4^wcm6/+	standard conditions	Fig. 4. from Sam et al., 2020
heart tube decreased length, abnormal	gata5^wcm8/wcm8; gata4^wcm6/+	standard conditions	Fig. 5. from Sam et al., 2020
heart looping process quality, abnormal	gata5^wcm8/wcm8; gata4^wcm6/+	standard conditions	Fig. 5. from Sam et al., 2020
heart tube linear, abnormal	gata5^wcm8/wcm8; gata4^wcm6/+	standard conditions	Fig. 5. from Sam et al., 2020
embryonic heart tube elongation process quality, abnormal	gata5^wcm8/wcm8; gata4^wcm6/+	standard conditions	Fig. 5. from Sam et al., 2020
heart primordium nkx2.5 expression decreased amount, abnormal	gata5^wcm8/wcm8; gata4^wcm6/+	standard conditions	Fig. 4. from Sam et al., 2020
heart tube absent, abnormal	gata5^wcm8/wcm8; gata4^wcm6/wcm6	standard conditions	Fig. 6. from Sam et al., 2020
cardiac muscle cell myl7 expression decreased amount, abnormal	gata5^wcm8/wcm8; gata4^wcm6/wcm6	standard conditions	Fig. 4. from Sam et al., 2020
heart tube morphology, ameliorated	gata5^wcm8/wcm8; gata4^wcm6/wcm6	standard conditions	Fig. 4. from Sam et al., 2020
heart primordium nkx2.5 expression decreased amount, abnormal	gata5^wcm8/wcm8; gata4^wcm6/wcm6	standard conditions	Fig. 4. from Sam et al., 2020
cardiac muscle cell myl7 expression absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/+	standard conditions	Fig. 3. from Sam et al., 2020
heart primordium nkx2.5 expression absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/+	standard conditions	Fig. 3. from Sam et al., 2020
cardiac muscle cell absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/wcm7	standard conditions	Fig. 3. from Sam et al., 2020
heart tube absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/wcm7	standard conditions	Fig. 3. from Sam et al., 2020
cardiac muscle cell myl7 expression absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/wcm7	standard conditions	Fig. 3. from Sam et al., 2020
heart primordium absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/wcm7	standard conditions	Fig. 3. from Sam et al., 2020
heart primordium nkx2.5 expression absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/wcm7	standard conditions	Fig. 3. from Sam et al., 2020
heart tube decreased thickness, abnormal	gata5^wcm8/+; gata6^wcm7/+; gata4^wcm6/+	standard conditions	Fig. 6. from Sam et al., 2020
heart tube straight, abnormal	gata5^wcm8/+; gata6^wcm7/+; gata4^wcm6/+	standard conditions	Fig. 6. from Sam et al., 2020
heart tube absent, abnormal	gata5^wcm8/+; gata6^wcm7/wcm7; gata4^wcm6/wcm6	standard conditions	Fig. 6. from Sam et al., 2020
heart tube absent, abnormal	gata5^wcm8/wcm8; gata6^wcm7/+; gata4^wcm6/wcm6	standard conditions	Fig. 6. from Sam et al., 2020

Citations