FIGURE

Fig. 5

ID

ZDB-FIG-190723-330

Publication

Veloso et al., 2019 - Dephosphorylation of HDAC4 by PP2A-Bδ unravels a new role for the HDAC4/MEF2 axis in myoblast fusion

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Fig. 5

PP2A-Bδ holoenzyme regulates cytoskeleton dynamics via the RhoGTPase Rac1.

a Rac1 activity was measured by GST pull-down assay in control (shCTL) and Bδ-knocked down (shBδ) C2C12 myoblasts at the indicated time points during the differentiation process. Values are mean ± SD from at least three experiments. Unpaired t-test, two-tailed, *P < 0.05. b Western blot analysis of phospho-Pak1,2 (α-pPAK1,2) and total Pak 1,2 (α-PAK1,2) in control (shCTL) and Bδ-knocked down (shBδ) C2C12 myoblasts at the indicated time points during the differentiation process. Images are representative of two independent experiments. c Confocal analysis of F-actin (phalloidin, gray) in control (shCTL) and Bδ-knocked down (shBδ) C2C12 myoblasts treated ( + ) or not (−) with a Rac1 inhibitor (NSC23766). Imaging was done at the indicated time points during the differentiation process. Representative cells from two independent experiments are shown. Scale bars are 10 µm. d Major/minor cell axis ratio in control Bδ-KD (shBδ) C2C12 myoblasts treated or not with the NSC23766 Rac1 inhibitor. Analysis was performed at day 2 during the differentiation process. Cells from 2 independent experiments for each condition were analyzed (n = 570 for shBδ and n = 585 for shBδ + Rac inhibitor). Mann–Witney’s test ****P < 0.0001. e Analysis of Abl, CrkII, and FAK activation by western blot in control (shCTL) and Bδ-KD (shBδ) C2C12 myoblasts during differentiation using antibodies against the phosphorylated forms of their activation sites, i.e., Y412 (α-pAbl (Y412)), (α-pCrkII (Y221)), and (α-pFAK (S273)), respectively. In total, Abl, CrkII, and FAK were used as loading controls. Images are representative of at least two experiments

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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